Instrument: Illumina HiSeq 2500
Strategy: ncRNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: SINGLE
Construction protocol: Total RNA, including the small RNA fraction, was obtained by organic extraction followed by miRNeasy purification (Qiagen). RNA-Seq Illumina libraries for small RNA profiling were prepared from 1 ug of total RNA using the TruSeq Small RNA Sample Prep Kit (Illumina).